In summary, this study demonstrates that the effects of a fructose diet on metabolism and bp are dependent on sex. In wistar rats, 21 weeks of fructose supplementation induced a metabolic syndrome hypertension, insulin resistance, and dyslipidemia but not. Resveratrol decreases fructoseinduced oxidative nadph. Fructoseinduced insulin resistance and hypertension in rats ishun hwang, helen ho, brian b.
The effect of hydroalcoholic celery apiumgraveolens. Fructoseinduced insulin resistance and hypertension in rats. Relative liver weight and plasma concentrations of glucose and triglycerides. Cellular mechanisms of insulin resistance in rats with. Further studies will be required to assess the contribution of insulin receptor abnormalities to the development of the hypertensive response to chronic fructose feeding in rats. Hypertension associated with fructose and high salt. To ascertain the role of glut5 in fructose absorption in the intestine in vivo and fructoseinduced hypertension, mice lacking the glut5 gene glut5 were placed on either high fructose or normal diet and compared with their wildtype littermates glut5. The average plasma fructose concentration in the fructosefed mice was 1550.
The protective role of amla emblica officinalis gaertn. Using a rat model with fructoseinduced metabolic syndrome, the effect of gravinol was investigated. It also promotes excretion of enterobacteriales generated by the dietary intervention. In parallel studies, a chloridefree diet blocked fructoseinduced hypertension in sprague dawley rats. Fructosefed rats and mice show moderate hypertension and glucose intolerance, associated with increased levels of.
Fructose drinking rats had increased blood pressure and impaired acetylcholineinduced relaxation of the thoracic aorta in comparison with control rats drinking just tap water. Mangiferin diminishes chronic fructose overconsumptioninduced cardiac fibrosis in spontaneous hypertension rats ling yao 1, chunli li2, ting chen1, weiguo cao, johji yamahara3, yuhao li1,4, yunmei zhang5, jianwei wang1 1chongqing key laboratory of traditional chinese medicine for prevention and cure of metabolic diseases. We hypothesized that a 28week diet rich in fat, fructose. The addition of clonidine to the drinking water inhibited fructoseinduced hypertension, but not the increase in plasma insulin or triglyceride concentration seen in fructosefed rats. Dietary lipoic acid supplementation prevents fructose. Rats with fructoseinduced hypertension were treated by oral gavage with.
Metabolic syndrome and hypertension resulting from fructose. Slc2a5glut5isessentialfortheabsorptionoffructosein. Changes in sbp of control and fructosefed rats, with or without aliskiren treatment, are shown in figure 1. Here we investigate the effects of fructose feeding and of varying salt intake on blood pressure, glucose tolerance, plasma renin activity, and tissue angiotensinogen, renin, and at 1 receptor mrna.
Effect of aqueous extract of defatted flaxseeds linum. In these rats, restoration of normal insulin sensitivity by regular exercise6 and normalization of plasma insulin levels by. Resveratrol decreases the bp in these fructoseinduced hypertensive rats through the activation of ampk, which, by. Forty wistar rats were randomly divided into five groups and the. These results suggest that aldehydes may be the cause of fructose. To determine if hypertension could be produced in normal rats by feeding them a fructoseenriched diet, spraguedawley rats were fed either normal chow or a. Inulin supplementation prevents high fructose dietinduced. Serum uric acid remained unchanged in animals on increased fructose intake with hypertension. Our results suggest that in the nts, fructose induces nadph oxidase activity and reduces sod2 activity, which contributes to increase bp in fructosefed rats. Fructoseinduced hypertension in rats is concentration and duration. Blood pressure increased significantly p fructosefed rats receiving the highsalt diet. Cellular mechanisms of insulin resistance in rats with fructose.
In fructosefed rats administration of allopurinol lowered uric acid levels and significantly reduced blood pressure, improved basal and stimulated insulin levels, corrected the hypertriglyceridemia, and prevented the weight gain. Because fructose cannot be used directly as a source of energy, the metabolisms of. Injection of activated th17 lymphocytes isolated from fructosefed ss rats induced hypertension via increase of serum il17a in only recipient ss rat. Resveratrol may activate ampk by eliminating ros production in the nts of rats with fructose. Male wistar rats were fed a 65% fructose diet and administered 10 or 20 mg of gravinolkg of body weightday for 2 weeks. Fructoseinduced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats laura g. Effect of eclipta alba on fructose induced hypertension in. The activation of ras by high fructose is demonstrated by the increase of plasma ang ii levels after 26 weeks of 6066% fructose intake in rats. We investigated the antihypertensive effect of a methanol extract me of fenugreek seeds and its methanol fraction mf in deoxycorticosterone acetate docasaltinduced and fructoseinduced hypertensive rats.
These data highlight the potential utility of enos gene therapy in the treatment of hypertension and insulin resistance. Highlevel fructose feeding led to hyperglycemia, hyperlipidemia, hypertriglyceridemia, and hypertension. Rats were fed a 66% fructose diet containing either low 0. Free radical scavenging reverses fructoseinduced saltsensitive hypertension zachary p zenner, kevin l gordish, william h beierwaltes department of internal medicine, hypertension and vascular research division, henry ford hospital, detroit, mi, usa abstract. Feeding a highfructose diet induces hypertension and insulinresistance in spraguedawley rats. Antihypertensive effect of trigonella foenumgreacum. Dietary lipoic acid supplementation prevents fructoseinduced hypertension in rats article in nutrition metabolism and cardiovascular diseases 106. Increased endothelial nitricoxide synthase expression.
The tissue reninangiotensin system in rats with fructose. Metabolic syndrome signs in wistar rats submitted to. It is also confirmed by the usage of antagonists of angiotensin receptor or renin to attenuate fructoseinduced hypertension 14,15. Original article mangiferin diminishes chronic fructose. The mechanisms underlying fructoseinduced hypertension. Gut dysbiosis contribute to high fructose induced salt. Highfructose intake induced hypertension via the activation of pathogenic th17 lymphocytes in ss but not sr rats. Isops markers of oxidative stress, steatosis, and liver dags, without significantly affecting perigonadal fat deposition, and impaired glucose tolerance. Systolic blood pressure in rats model of hypertension.
Masoprocol lowers blood pressure in rats with fructoseinduced. Hypertension was induced by administration of 10% wv solution of fructose orally in drinking water. Amyrin attenuates high fructose dietinduced metabolic. Pdf dietary vitamin e and c supplementation prevents. Fructoseinduced hypertension in rats is concentration and durationdependent. The rats were treated with 5%, 10%, or 20% fructose in drinking water. Cardiovascular effects of highfructose intake in rats. The role of the baroreflex and parasympathetic nervous. The role of salt in the pathogenesis of fructoseinduced. Free radical scavenging reverses fructoseinduced salt. Female rats are protected against fructoseinduced changes. In fact, a preclinical study demonstrated a direct involvement of increased rsna in fructoseinduced saltsensitive hypertension in conscious rats chronically instrumented with nerve telemetry devices. Female rats are protected against fructoseinduced hypertension, unlike their male counterparts, and the mechanisms responsible for this protection appear to.
Fructose induced hypertension in rats is concentration and durationdependent soter dai and john h. Fructoseinduced hypertension in rats is concentration and. Abstract objective fructose feeding induces hypertension, insulinresistance and hypertriglyceridemia in spraguedawley rats. Several lines of evidence suggest that insulin resistance and hyperinsulinemia may play a pathogenic role in the development. Metabolic syndrome and hypertension resulting from. Fructose, a sweeter alternative to glucose, is usually used in smaller amounts than glucose.
Activated pathogenic th17 lymphocytes induce hypertension. Studies in rats have confirmed this speculation by showing that high salt intake enhances and low salt intake blunts the magnitude of fructoseinduced. Female rats are protected against fructoseinduced changes in metabolism and blood pressure denise galipeau,1 subodh verma,2 and john h. In this model, feeding normal sprague dawley rats a fructoseenriched diet 66% fructose results in hyperinsulinemia, insulin resistance and hypertension independent of obesity. Indeed, several studies have pointed to the adverse cardiac and metabolic effects of a high fructose diet in animal models. Mechanisms of hypertension in hyperinsulinemic and insulin. Sudesh vasdev, a vicki gill, a sushil parai, a vereesh gadag b. Summary to determine if hypertension could be produced in normal rats by feeding them a. High salt intake in rats on 20% fructose, but not glucose, resulted in a 41% increase in rsna after 1 week. It is wellestablished that baroreflex sensitivity is essential for blood pressure control, and also plays a key role in the modulation of diseaseinduced metabolic alterations.
Fructoseinduced metabolic syndrome is associated with. Fructose feeding can also cause insulin resistance and hyperinsulinemia in normal r a t s. Taurine prevents hypertension and increases exercise. In conclusion, this study indicates a possible cellular mechanism for the reduced sensitivity to insulin present in rats with fructoseinduced hypertension. Request pdf effect of eclipta alba on fructose induced hypertension in albino rats objective hypertension is a common disorder rising in incidence once established treatment is obligatory. Pdf the present study determined the most suitable concentration and duration of fructose treatment for inducing hypertension in wistar rats. The 16s rrna sequencing for fecal samples was assessed and blood pressure was recorded. If fructose stimulates salt absorption in the intestine and kidney, then it is reasonable to ask whether varying the amount of dietary salt has any impact on fructoseinduced hypertension. Research paper prevention of dexamethasone and fructoseinduced insulin prevention of dexamethasone and fructoseinduced insulin resistance in rats by sh01d, a herbal preparation resistance in rats by sh01d, a herbal preparation. Fructoseinduced hypertension has been shown to be attenuated in both glut5 and pat1 knockout mice. The present study determined the most suitable concentration and duration of fructose treatment for inducing hypertension in wistar rats. Gravinol ameliorates highfructoseinduced metabolic. In addition, hyperinsulinemia and hypertriglyceridemia were associated with hypertension in fructosefed rats. We have previously reported that a moderate dietary supplementation of 20% fructose but not glucose leads to a saltsensitive.
Dietary vitamin e and c supplementation prevents fructose. The correlation between fructoseinduced hypertension and hyperinsulinemia was also evaluated. Nts, thereby reducing bp in rats with fructoseinduced hypertension. Male wistar rats were fed a highfructose 65 % diet or standard chow for 1 week, and treated with an ethyl acetate etoac extract of amla, a polyphenolrich fraction, at 10 or 20 mgkg body weight per d, or vehicle, for 2 weeks. The results of this study cumulatively indicate that gene therapy with human enos decreased fructoseinduced hypertension and insulin resistance in rats and suggest potential signaling pathways that mediate these effects. The relation between fructoseinduced metabolic syndrome and. Antihypertensive effect of silymarin on fructose induced. The pathogenesis of fructoseinduced hypertension is dubious and. Mcneil1 division of pharmacology and toxicology, faculty of pharmaceutical sciences. The greatest changes, including increases in blood pressure, fluid intake, and plasma levels. Longterm dietinduced hypertension in rats is associated. Recently, the role of fructoseinduced hyperuricemia in the development of metabolic syndrome was examined. Pdf fructoseinduced hypertension in rats is concentration and. In wistar rats, 21 weeks of fructose supplementation induced a metabolic syndrome hypertension, insulin resistance, and dyslipidemia but not t2d.
Sef was given orally once daily at the doses of 50,100 and 200mgkg. The mechanisms of fructoseinduced hypertension are as yet unknown. Metabolic syndrome was induced in rats over 8 wk by either a highfructose diet 60%, f60, n 7 or by adding fructose to drinking water 10%, f10, n 7. This pdf is available for free download from a site hosted by medknow publications. The fructoseinduced hypertensive fh rat is a dietinduced model of mild hhypertension. The magnitude of blood pressure of the c and ce did not alter during the period of experiment figure 1.
Role of aldehydes in fructose induced hypertension. We investigated the effects of amla emblica officinalis gaertn. To determine whether fructoseinduced hypertension is dependent on dietary salt content, rats were fed standard rat chow and a fructoseenriched diet with low and high sodium chloride concentrations. Fructose feeding induces a rise in blood pressure in normal rats and is associated with insulin resistance, hyperinsulinemia and hypertriglyceridemia. The present study was carried out to investigate the antihypertensive effect of silymarin 300 mgkg and 500 mgkg, p. Cardiovascular autonomic dysfunction and oxidative stress. Fructoseinduced hypertension in rats is concentration. Sbp increased significantly in rats receiving the normal diet and highsalt diet. Reaven summary to determine if hypertension could be produced in normal rats by feeding them a. Hypertension developed was confirmed by systolic blood pressure sbp measured after 2 weeks of administration. Thus, the metabolic changes associated with fructoseinduced hypertension are unlikely to be secondary to an increase in sympathetic activity. Male wistar rats were randomly distributed into different groups. Mcneill1 1division of pharmacology and toxicology, university of british columbia, vancouver, british columbia v6t 1z3. Pdf fructoseinduced insulin resistance and hypertension.
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